Improving recombinant eukaryotic membrane protein yields inPichia pastoris: The importance of codon optimization and clone selection
نویسندگان
چکیده
منابع مشابه
Targeted Deletion of Los1 Homologue Affects the Production of a Recombinant Model Protein in Pichia pastoris
Background: The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which i...
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BACKGROUND The increasing availability of 'omics' databases provide important platforms for yeast engineering strategies since they offer a lot of information on the physiology of the cells under diverse growth conditions, including environmental stresses. Notably, only a few of these approaches have considered a performance under recombinant protein production conditions. Recently, we have ide...
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Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...
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ژورنال
عنوان ژورنال: Molecular Membrane Biology
سال: 2011
ISSN: 0968-7688,1464-5203
DOI: 10.3109/09687688.2011.602219